![]() ![]() ![]() Please see the Kit Components List to determine kit components. Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. CLEARLY Stained Protein Ladder is sufficient for 50 lanes. QuantityĬLEARLY Protein Ladder (Unstained) is sufficient for 100 lanes. They remain stable for up to 50 freeze-thaw cycles. These products are stable for 6 months when stored at 4☌. Ensure that the ladder is completely thawed before removing an aliquot for loading.Pre-stained ladder (Cat.# 3454): Load 10 µl per lane.Unstained ladder (Cat.# 3453): Load 5 µl per lane.*Reference band (four-fold greater intensity relative to the other bands) SDS-PAGE recommendations CLEARLY Stained Protein Ladder (Cat.# 3454A and 3454B).*Reference band (four-fold greater intensity relative to the other bands) CLEARLY Protein Ladder (Unstained) (Cat.# 3453A and 3453B).# 3453A for complete product documentation and resources. In this case, this ladder is sufficient for 100 lanes. Cat. 5 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. This ladder is pre-mixed with loading buffer and ready to load. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. When this ladder is analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, it is resolved into quite clearly identifiable bands from 10 to 250 kDa. Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel Migration pattern of CLEARLY Stained Protein Ladder (Cat.# 3454A) on an 12% SDS-PAGE gel.ĬLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins. *: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. # 3454A for complete product documentation and resources. *: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. Cat. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting. In this case, this ladder is sufficient for 50 lanes. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. In low-percentage gels (100 kDa) proteins.CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins. See the table provided for migration patterns in different electrophoresis conditions. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. ![]() Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. The loading volume should be doubled for 1.5 mm thick gels.ġ. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. Use the same volumes for Western blotting. Load the following volumes of the ladder on an SDS-polyacrylamide gel: Mix gently, but thoroughly, to ensure the solution is homogeneous.ģ. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. ![]()
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